100x To 1x Dilution Calculator

Dilution Formula:

\[ DF = \frac{v2}{v1} \]

Dilution Factor (DF):

Volume of Diluted Solution (v2):

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1. What is 100x to 1x Dilution?

A 100x to 1x dilution means taking a concentrated stock solution (100x) and diluting it to a working concentration (1x). This is commonly used in laboratory settings for preparing solutions from concentrated stocks.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

\[ DF = \frac{v2}{v1} \]

Where:

\( DF \) — Dilution Factor (100 for 100x to 1x dilution)
\( v1 \) — Volume of stock solution (mL)
\( v2 \) — Volume of diluted solution (mL)

Explanation: For a 100x to 1x dilution, you need to dilute 1 part of the 100x stock solution with 99 parts of diluent to achieve the final 1x concentration.

3. Importance of Dilution Calculation

Details: Accurate dilution calculations are essential in laboratory work, pharmaceutical preparations, and various scientific experiments to ensure proper concentration of solutions for accurate results and reproducibility.

4. Using the Calculator

Tips: Enter the volume of your stock solution in mL. The calculator will determine the required volume of diluted solution needed for a 100x to 1x dilution.

5. Frequently Asked Questions (FAQ)

Q1: What does 100x concentration mean?
A: A 100x concentration means the stock solution is 100 times more concentrated than the working (1x) solution.

Q2: How do I prepare a 100x to 1x dilution?
A: Mix 1 part of the 100x stock solution with 99 parts of the appropriate diluent (usually water or buffer).

Q3: Can I use this calculator for other dilution factors?
A: This calculator is specifically designed for 100x to 1x dilutions. For other dilution factors, you would need to adjust the formula accordingly.

Q4: What if I need a different final volume?
A: Enter the desired volume of your stock solution, and the calculator will tell you the total volume of diluted solution you'll obtain.

Q5: Are there any limitations to this calculation?
A: This calculation assumes perfect mixing and that the diluent volume equals the difference between v2 and v1. For very precise work, consider the effects of temperature and potential volume changes upon mixing.