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nM Calculation Formula:
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The nM from ng/μL and length calculation converts DNA concentration from mass/volume units (ng/μL) to molar concentration (nM), which is essential for many molecular biology applications where equimolar amounts of DNA are required.
The calculator uses the formula:
Where:
Explanation: This formula accounts for the fact that the molar concentration of DNA depends on both its mass concentration and length, as longer DNA molecules have higher molecular weights.
Details: Converting to molar units (nM) is crucial for applications like PCR, sequencing, and cloning, where equimolar amounts of different DNA fragments are needed for proper stoichiometry and reaction efficiency.
Tips: Enter DNA concentration in ng/μL and length in base pairs. Both values must be positive numbers. The calculator will provide the equivalent concentration in nM.
Q1: Why is the conversion factor 1.52?
A: The factor 1.52 comes from the average molecular weight of a DNA base pair (660 g/mol) and unit conversions from ng/μL to nM.
Q2: Does this work for both double-stranded and single-stranded DNA?
A: This formula is specifically designed for double-stranded DNA. For single-stranded DNA, a different conversion factor would be needed.
Q3: Can I use this for RNA concentration conversion?
A: No, RNA has a different average molecular weight per base. A different conversion factor would be required for RNA.
Q4: How accurate is this conversion?
A: The conversion is based on average molecular weights and provides a good estimate for most applications, though exact values may vary slightly based on DNA sequence composition.
Q5: What if my DNA length is in kb instead of bp?
A: Convert kb to bp first (1 kb = 1000 bp) before using the calculator for accurate results.